Optimization and validation of spectrophotometric determination of glucosamine in dosage Form with sodium 1,2-naphthoquinone-4-sulphonate

Document Type : Original Article

Authors

1 Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt

2 Sustainable Chemistry and Material Resources, Institute of Sustainable and Environmental Chemistry, Leuphana University Lüneburg, C13, DE-21335 Lüneburg, Germany

3 Department of Pharmacology and Toxicology, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia

Abstract

A rapid, sensitive and simple optimized spectrophotometric method is developed for the determination of Glucosamine (GL) based on reaction of GL with sodium 1,2-naphthoquinone-4-sulphonate (NQS) in alkaline medium forming orange colored product which measured at 451 nm. The method optimization was essential as many procedural parameters influenced the efficiency and reproducibility of the derivatization process including the reaction time, reaction temperature,NQS concentration, alkali concentration and solvents. The objective of our study was to optimize and validate a spectrophotometric method using the reaction of GL with NQS for determining GL in dosage form. The factors that may influence the efficiency and reproducibility of the derivatization process were identified and optimized. The proposed method was validated and successfully applied for the analysis of GL in dosage form. Statistical comparison of the results of the proposed method with the reference method showed excellent agreement and proved that no significant difference in the accuracy and precision. The developed method is easy to use, accurate and least cost-effective for routine studies relative to HPLC and other techniques

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