Spectral resolving of propofol and benzyl alcohol mixture

Ramadan, Hesham Sameh; Abdel Salam, Randa; Hadad, Ghada; Belal, Fathalla; Salim, Mohamed M.

doi:10.21608/rpbs.2023.237410.1243

Spectral resolving of propofol and benzyl alcohol mixture

Document Type : Original Article

Authors

¹ Analytical chemistry, faculty of pharmacy, suez canal university

² pharmaceutical analytical chemistry, faculty of pharmacy, Suez Canal University,Ismailia, Egypt

³ pharmaceutical analytical chemistry, faculty of pharmacy, Suez Canal University, Ismailia, Egypt

⁴ pharmaceutical analytucal chemistry, faculty of Pharmacy, Mansoura University

⁵ Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt

10.21608/rpbs.2023.237410.1243

Abstract

Two simple spectrophotometric methods are proposed for the spectral resolving of propofol and benzyl alcohol in their synthetic mixture. 1st derivative spectrophotometry (Method I) is used for the determination of propofol at 324.1 nm with no significant interaction from benzyl alcohol, while spectral subtraction followed by 1st derivative spectrophotometry (Method II) is used for the determination of benzyl alcohol at 308.15 nm, successfully eliminating the interference from propofol. Both methods were with acceptable linearity (20-80 μg/mL and 3-12 μg/mL for propofol in Method I and benzyl alcohol in Method II, respectively). The standard deviations were 0.00069 and 0.00293 for method I and II, respectively and the correlation coefficient (r) is equal to 0.9999 for both methods, indicating excellent linearity. The reported methods show promise for future application on their coformulated dosage forms, enabling successful determination of both compounds of interest, which can be useful for routine quality control of monitoring maximum concentration of benzyl alcohol used as a preservative.

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