An Approach for Developing a Method for Biosurfactant Activity Detection

Document Type : Mini-reviews

Authors

1 Department of Microbiology and Immunology, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt.

2 Department of Microbiology and Immunology, Faculty of Pharmacy, , Suez Canal University, Ismailia, Egypt.

Abstract

Detection  of  biosurfactant  activity  have  been  proposed  Previously  by several studies either quantitatively or qualitatively. Quantitative methods give precise measurement of the surface tension using accurate devices while  qualitative  measurements  are  considered  screening  methods  for biosurfactant activity detection with lower precision. Due to the high cost of the direct measurement devices and in some cases the unavailability of devices,  qualitative  methods  remain  the  most  common  used  tests  for screening of the surface-active properties. The need for an accurate, cheap method was the reason for this trial of developing a photometric method for  a  rapid,  accurate  biosurfactant  activity  detection.  “α-naphthol- butanol”  method  is  based  on  measuring  the  photometric  activity  of  a sparingly soluble compound “α-naphthol” at its λmax. Formed emulsion of oily  phase  “containing  α-naphthol”  and  aqueous  phase  is  based  on  the surfactant  concentration  and  the  surface  tension  of  the  solution  being tested. This method passed the first and second confirmatory tests but, in the  third  trial  it  failed.  Knowing  the  drawbacks  of  this  method  we  can manage to develop a promising modification.  

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Records of Pharmaceutical and Biomedical Sciences
Volume 8, Issue 2
Pharmacognosy-Microbiology
2024
Pages 106-114

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