Quantitative determination of captopril, perindopril erbumine, moexipril hydrochloride, and ramipril in bulk and pharmaceutical preparations by high performance liquid chromatography

Document Type : Original Article

Authors

1 Medicinal Chemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia, 41522, Egypt.

2 Medicinal Chemistry Department, Faculty of Pharmacy, University of Suez Canal, Ismailia 41522, Egypt

3 Medicinal Chemistry Department, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt

Abstract

  A high-performance liquid chromatographic method was developed for simultaneous determination of four ACE-inhibitors: captopril (CAP), perindopril erbumine (PER), moexipril hydrochloride (MOEX), and ramipril (RAM) in pharmaceutical formulations. The chromatographic separation was performed on Shim-pack cyanopropyl column with a mobile phase consisting of methanol-10mM ammonium acetate buffer (pH 6.0) in a ratio of (40: 60, v/v) at flow rate 1 ml min-1. the analysis was performed at ambient temperature using UV detector setting at 210 nm. All ACE-inhibitors were separated within seven min. The calibration curves were linear (r ≥ 0.9994) over a concentration range from 5 to 50 µg ml-1. the method was successfully applied to commercially available pharmaceutical preparations. The validity of the method was examined comparing the results obtained with official or published methods.

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